Analysis of Cordyceps by multi-column liquid chromatography

Analysis of Cordyceps by multi-column liquid chromatography

Cordyceps is a famous traditional Chinese medicine (TCM) that has been used in China for hundreds of years. In the present study a multi-column liquid chromatography (MC-LC) system was developed for the qualitative analysis of macromolecules and micromolecules in Cordyceps. The MC-LC system includes a size exclusion pre-column, a size exclusion column (SEC) and a reversed phase column (RP) whic...

Column Liquid Chromatography

Purification of pituitary autoantigen by column liquid chromatography and chromatofocusing

INTRODUCTION Pituitary autoantibodies can be determined both in patients with pituitary disease as well as patients with autoimmune endocrine diseases. The purpose of the study was to isolate and purify pituitary autoantigen using sera of patients and the microsomal fraction of the pituitary. MATERIAL AND METHODS To isolate a pituitary autoantigen, patient sera were used, which showed a stron...

Column liquid chromatography: equipment and instrumentation.

Injectors/Autosamplers 2815 Temperature Effects In LC 2815 Sample Preparation/Derivatization 2816 Microchip Technology in LC 2816 Automation 2817 Other Articles of Interest 2817 Columns 2817 Reviews 2817 Columns Materials, Effects, and Packing 2817 Supports 2817 Stationary Phases 2818 Ion-Exchange Phases 2819 Monolithic Columns 2819 Molecular Imprinted Polymer (MIP) Phases 2819 Tunable Stationa...

Quantitative analysis of three chiral pesticide enantiomers by high-performance column liquid chromatography.

Methods for the enantiomeric quantitative determination of 3 chiral pesticides, paclobutrazol, myclobutanil, and uniconazole, and their residues in soil and water are reported. An effective chiral high-performance liquid chromatographic (HPLC)-UV method using an amylose-tris(3,5-dimethylphenylcarbamate; AD) column was developed for resolving the enantiomers and quantitative determination. The e...